Topping-off technique has been proposed to prevent adjacent-segment degeneration/disease following spine fusion surgery. Nevertheless, few studies have investigated biomechanics of the fusion surgery with topping-off device under whole-body vibration (WBV). This biomechanical study aimed to investigate the vibration characteristics of human lumbar spine after topping-off surgery, and also to evaluate the effect of bony fusion on spine biomechanics. Based on a healthy finite-element model of lumbosacral spine (L1-sacrum), the models of topping-off surgery before and after bony fusion were developed. The simulated surgical procedures consisted of interbody fusion with rigid stabilizer at L4-L5 segment (rigid fusion) and dynamic stabilizer at degenerated L3-L4 segment. An interspinous implant, Device for Intervertebral Assisted Motion (DIAM, Medtronic Inc., Minnesota, USA), was used as the dynamic stabilizer. The stress responses of spine segments and implants under a vertical cyclic load were calculated and analyzed. The results showed that compared with rigid fusion alone, the topping-off technique significantly decreased disc stress at transition segment (L3-L4) as expected, and resulted in a slight increase in disc stress at its supra-adjacent segment (L2-L3). It indicated that the topping-off stabilization using DIAM might provide a good tradeoff between protection of transition segment and deterioration of its supra-adjacent segment during WBV. Also, it was found that bony fusion decreased stress in L4 inferior endplate and rigid stabilizer but had nearly no effect on stress in DIAM and L3-L4 disc, which was helpful to determine the biomechanical differences before and after bony fusion.