Robotic assistance technologies are being incorporated into minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF) to minimize radiation exposure to the patient and operating staff. However, they introduce new issues including increased operating time and difficult incorporation into surgical workflow. This study, conducted with 42 patients under the care of one neurosurgeon in Sydney, Australia, investigates the operating time increase with three different robotic modalities, and the learning curves they pose to the surgeon. Between the comparable modalities of freehand MIS-TLIF and Mazor Renaissance® CT to Fluoro, there was a significant increase in time from patient draping to insertion of the final K-wire (p = 0.0019), and a non-significant increase in time per K-wire (p = 0.55) using Mazor Renaissance®. Comparing the ROSA® and Mazor Renaissance® Scan and Plan, there were significant increases in drape to final K-wire time and time per K-wire using ROSA® assistance (p = 0.000068 and p = 0.011). ROSA® also had a steeper learning curve compared to both Mazor Renaissance® modalities, which were similar. Our study shows that Mazor Renaissance® modalities are superior to ROSA® in minimizing extra operating time, and also have easier learning curves; however, both modalities increase operating time compared with freehand MIS-TLIF. This study, to our knowledge, is the first to compare multiple robotic techniques in MIS-TLIF. Though these results highlight important differences between robotic modalities that are crucial for spinal surgeons to understand, the low sample size and variability in data reveal the need for larger, multi-centre studies in this field.